Development and validation of a collagen-based scaffold for vascular tissue engineering

Habermehl, Jason
Maître ès sciences (M.Sc.)
Université Laval
FACULTÉ DES SCIENCES ET DE GÉNIE
Maîtrise en génie de la métallurgie

Directeur(trice) de recherche : Mantovani, Diego

Co directeur(trice) de recherche : Laroche, Gaétan

2005-12

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Figure 1.1 : The cardiovascular system
Figure 1.2 : The physiology of the typical artery and vein.
Figure 1.3 : The structure of collagen. From the tropocollagen helix to the fiber
Figure 1.4 : Tissue engineering. The basics.
Figure 2.1 : Collagen cell-seeded gel approach to vascular tissue regeneration
Figure 2.2 : Strategy of the research project
Figure 2.3 : Custom-made cutter for thin film mechanical testing sample preparation.
Figure 2.4 : Custom-made tube mandrel set-ups.
Figure 2.5 : Custom-made rotation device for cylindrical gels. A: isometric view, B: top view, C: front view.
Figure 2.6 : Gel contraction seeded with smooth muscle cells (SMC) and fibroblasts (FB).
Figure 2.7 : Typical collagen sell-seeded tube obtained with the gas-exchange tube-mandrel system
Figure 2.8 : Masson’s trichrome on a longitudinal a) and transversal b) section of tubular collagen gel scaffold seeded with smooth muscle cells after 1 week maturation.
Figure 2.9 : The various phases of the cell cycle
Figure 2.10 : Platelet adhesion on collagen gel seeded with smooth muscle cells without (A-C) and with (B-D) endothelial cells layer on the surface.
Figure 2.11 : Compliance apparatus
Figure 2.12 : Collagen tubular gels in the compliance apparatus. A: before dilatation, B: after dilatation
Figure 2.13 : Tissue engineered blood vessel (TEBV) bioreactor module.
Figure 3.1 : Curve fitting of the Amide I infrared peak of collagen samples A and B before (a and b) and after neutralization (c and d).
Figure 3.2 : Tensile properties of collagen films a) ultimate strength b) elastic modulus c) maximum elongation.
Figure 3.3 : Representative Stress-Strain curves of films made of acidic and neutral collagen films.
Figure 3.4 : Scanning electron microscopy (SEM) analyses of NIH 3T3 cells after 1 week on neutral collagen A (a) and neutral collagen B (b).
Figure 3.5 : MTT assay testing cytotoxicity of different collagens on NIH 3T3 after 24h and 1 week.
Figure 3.6 : Haemoglobin free test on collagen, Teflon and glass.
Figure 3.7 : Fibrin formation.
Figure 3.8 : Thrombogenicity Index.
Figure 3.9 : SEM analyses of platelets on collagen film.
Figure 3.10 : SEM analyses of endothelial cell (a) and smooth muscle cell (b) morphology after 24 hours of contact with collagen films.
Figure 3.11 : MTT results on endothelial cells (a) and smooth muscle cells (b).

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